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Transposable element

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"Mobile DNA" redirects here. For the academic journal, see Mobile DNA (journal).

A bacterial DNA transposon

A transposable element (TE), also transposon, or jumping gene, is a type of mobile genetic element, a nucleic acid sequence in DNA that can change its position within a genome, sometimes creating or reversing mutations and altering the cell's genetic identity and genome size.[1][2]

Transposition often results in duplication of the same genetic material. The discovery of mobile genetic elements earned Barbara McClintock a Nobel Prize in 1983.[3] Further research into transposons has potential for use in gene therapy, and the finding of new drug targets in personalized medicine. The vast number of variables in the transposon makes data analytics difficult but combined with other sequencing technologies significant advances may be made in the understanding and treatment of disease.[4]

Transposable elements make up about half of the genome in a eukaryotic cell, accounting for much of human genetic diversity.[4] Although TEs are selfish genetic elements, many are important in genome function and evolution.[5] Transposons are also very useful to researchers as a means to alter DNA inside a living organism.

There are at least two classes of TEs: Class I TEs or retrotransposons generally function via reverse transcription, while Class II TEs or DNA transposons encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins.[6]

Discovery by Barbara McClintock

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Barbara McClintock discovered the first TEs in maize (Zea mays) at the Cold Spring Harbor Laboratory in New York. McClintock was experimenting with maize plants that had broken chromosomes.[7]

In the winter of 1944–1945, McClintock planted corn kernels that were self-pollinated, meaning that the silk (style) of the flower received pollen from its own anther.[7] These kernels came from a long line of plants that had been self-pollinated, causing broken arms on the end of their ninth chromosomes.[7] As the maize plants began to grow, McClintock noted unusual color patterns on the leaves.[7] For example, one leaf had two albino patches of almost identical size, located side by side on the leaf.[7] McClintock hypothesized that during cell division certain cells lost genetic material, while others gained what they had lost.[8] However, when comparing the chromosomes of the current generation of plants with the parent generation, she found certain parts of the chromosome had switched position.[8] This refuted the popular genetic theory of the time that genes were fixed in their position on a chromosome. McClintock found that genes could not only move but they could also be turned on or off due to certain environmental conditions or during different stages of cell development.[8]

McClintock also showed that gene mutations could be reversed.[9] She presented her report on her findings in 1951, and published an article on her discoveries in Genetics in November 1953 entitled "Induction of Instability at Selected Loci in Maize".[10]

At the 1951 Cold Spring Harbor Symposium where she first publicized her findings, her talk was met with silence.[11] Her work was largely dismissed and ignored until the late 1960s–1970s when, after TEs were found in bacteria, it was rediscovered.[12] She was awarded a Nobel Prize in Physiology or Medicine in 1983 for her discovery of TEs, more than thirty years after her initial research.[13]

Classification

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Transposable elements represent one of several types of mobile genetic elements. TEs are assigned to one of two classes according to their mechanism of transposition, which can be described as either copy and paste (Class I TEs) or cut and paste (Class II TEs).[14]

Retrotransposon

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Main article: Retrotransposon

Class I TEs are copied in two stages: first, they are transcribed from DNA to RNA, and the RNA produced is then reverse transcribed to DNA. This copied DNA is then inserted back into the genome at a new position. The reverse transcription step is catalyzed by a reverse transcriptase, which is often encoded by the TE itself. The characteristics of retrotransposons are similar to retroviruses, such as HIV.

Despite the potential negative effects of retrotransposons, like inserting itself into the middle of a necessary DNA sequence, which can render important genes unusable, they are still essential to keep a species' ribosomal DNA intact over the generations, preventing infertility.[15]

Retrotransposons are commonly grouped into three main orders:

Retroviruses can also be considered TEs. For example, after the conversion of retroviral RNA into DNA inside a host cell, the newly produced retroviral DNA is integrated into the genome of the host cell. These integrated DNAs are termed proviruses. The provirus is a specialized form of eukaryotic retrotransposon, which can produce RNA intermediates that may leave the host cell and infect other cells. The transposition cycle of retroviruses has similarities to that of prokaryotic TEs, suggesting a distant relationship between the two.

DNA transposons

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Main article: DNA transposon
A. Structure of DNA transposons (Mariner type). Two inverted tandem repeats (TIR) flank the transposase gene. Two short tandem site duplications (TSD) are present on both sides of the insert.
B. Mechanism of transposition: Two transposases recognize and bind to TIR sequences, join and promote DNA double-strand cleavage. The DNA-transposase complex then inserts its DNA cargo at specific DNA motifs elsewhere in the genome, creating short TSDs upon integration.[16]

The cut-and-paste transposition mechanism of class II TEs does not involve an RNA intermediate. The transpositions are catalyzed by several transposase enzymes. Some transposases non-specifically bind to any target site in DNA, whereas others bind to specific target sequences. The transposase makes a staggered cut at the target site producing sticky ends, cuts out the DNA transposon and ligates it into the target site. A DNA polymerase fills in the resulting gaps from the sticky ends and DNA ligase closes the sugar-phosphate backbone. This results in target site duplication and the insertion sites of DNA transposons may be identified by short direct repeats (a staggered cut in the target DNA filled by DNA polymerase) followed by inverted repeats (which are important for the TE excision by transposase).

Cut-and-paste TEs may be duplicated if their transposition takes place during S phase of the cell cycle, when a donor site has already been replicated but a target site has not yet been replicated.[citation needed] Such duplications at the target site can result in gene duplication, which plays an important role in genomic evolution.[17]: 284 

Not all DNA transposons transpose through the cut-and-paste mechanism. In some cases, a replicative transposition is observed in which a transposon replicates itself to a new target site (e.g. helitron).

Class II TEs comprise less than 2% of the human genome, making the rest Class I.[18]

Autonomous and non-autonomous

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Transposition can be classified as either "autonomous" or "non-autonomous" in both Class I and Class II TEs. Autonomous TEs can move by themselves, whereas non-autonomous TEs require the presence of another TE to move. This is often because dependent TEs lack transposase (for Class II) or reverse transcriptase (for Class I).

Activator element (Ac) is an example of an autonomous TE, and dissociation elements (Ds) is an example of a non-autonomous TE. Without Ac, Ds is not able to transpose.

Class III

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Some researchers also identify a third class of transposable elements,[19] which has been described as "a grab-bag consisting of transposons that don't clearly fit into the other two categories".[20] Examples of such TEs are the Foldback (FB) elements of Drosophila melanogaster, the TU elements of Strongylocentrotus purpuratus, and Miniature Inverted-repeat Transposable Elements.[21][22]

Distribution

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Approximately 64% of the maize genome is made up of TEs,[23][24] as is 44% of the human genome,[25] and almost half of murine genomes.[26]

New discoveries of transposable elements have shown the exact distribution of TEs with respect to their transcription start sites (TSSs) and enhancers. A recent study found that a promoter contains 25% of regions that harbor TEs. It is known that older TEs are not found in TSS locations because TEs frequency starts as a function once there is a distance from the TSS. A possible theory for this is that TEs might interfere with the transcription pausing or the first-intro splicing.[27] Also as mentioned before, the presence of TEs closed by the TSS locations is correlated to their evolutionary age (number of different mutations that TEs can develop during the time).

Examples

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  • The first TEs were discovered in maize (Zea mays) by Barbara McClintock in 1948, for which she was later awarded a Nobel Prize. She noticed chromosomal insertions, deletions, and translocations caused by these elements. These changes in the genome could, for example, lead to a change in the color of corn kernels. About 64% of the maize genome consists of TEs.[24] The Ac/Ds system described by McClintock are Class II TEs. Transposition of Ac in tobacco has been demonstrated by B. Baker.[28]
  • In the pond microorganism, Oxytricha, TEs play such a critical role that when removed, the organism fails to develop.[29]
  • One family of TEs in the fruit fly Drosophila melanogaster are called P elements. They seem to have first appeared in the species only in the middle of the twentieth century; within the last 50 years, they spread through every population of the species. Gerald M. Rubin and Allan C. Spradling pioneered technology to use artificial P elements to insert genes into Drosophila by injecting the embryo.[30][31][32]
  • In bacteria, TEs usually carry an additional gene for functions other than transposition, often for antibiotic resistance. In bacteria, transposons can jump from chromosomal DNA to plasmid DNA and back, allowing for the transfer and permanent addition of genes such as those encoding antibiotic resistance (multi-antibiotic resistant bacterial strains can be generated in this way). Bacterial transposons of this type belong to the Tn family. When the transposable elements lack additional genes, they are known as insertion sequences.
  • In humans, the most common TE is the Alu sequence. It is approximately 300 bases long and can be found between 300,000 and one million times in the human genome. Alu alone is estimated to make up 15–17% of the human genome.[18]
  • Mariner-like elements are another prominent class of transposons found in multiple species, including humans. The Mariner transposon was first discovered by Jacobson and Hartl in Drosophila.[33] This Class II transposable element is known for its uncanny ability to be transmitted horizontally in many species.[34][35] There are an estimated 14,000 copies of Mariner in the human genome comprising 2.6 million base pairs.[36] The first mariner-element transposons outside of animals were found in Trichomonas vaginalis.[37]
  • Mu phage transposition is the best-known example of replicative transposition.
  • In Yeast genomes (Saccharomyces cerevisiae) there are five distinct retrotransposon families: Ty1, Ty2, Ty3, Ty4 and Ty5.[38]
  • A helitron is a TE found in eukaryotes that is thought to replicate by a rolling-circle mechanism.
  • In human embryos, two types of transposons combined to form noncoding RNA that catalyzes the development of stem cells. During the early stages of a fetus's growth, the embryo's inner cell mass expands as these stem cells enumerate. The increase of this type of cells is crucial, since stem cells later change form and give rise to all the cells in the body.
  • In peppered moths, a transposon in a gene called cortex caused the moths' wings to turn completely black. This change in coloration helped moths to blend in with ash and soot-covered areas during the Industrial Revolution.[39]
  • Aedes aegypti carries a large and diverse number of TEs. This analysis by Matthews et al. 2018 also suggests this is common to all mosquitoes.[40]

Negative effects

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Transposons have coexisted with eukaryotes for thousands of years and through their coexistence have become integrated in many organisms' genomes. Colloquially known as 'jumping genes', transposons can move within and between genomes allowing for this integration.

While there are many positive effects of transposons in their host eukaryotic genomes,[further explanation needed] there are some instances of mutagenic effects that TEs have on genomes leading to disease and malignant genetic alterations.[41]

Mechanisms of mutagenesis

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TEs are mutagens and due to the contribution to the formation of new cis-regulatory DNA elements that are connected to many transcription factors that are found in living cells; TEs can undergo many evolutionary mutations and alterations. These are often the causes of genetic disease, and gives the potential lethal effects of ectopic expression.[27]

TEs can damage the genome of their host cell in different ways:[41]

  • A transposon or a retrotransposon that inserts itself into a functional gene can disable that gene.
  • After a DNA transposon leaves a gene, the resulting gap may not be repaired correctly.
  • Multiple copies of the same sequence, such as Alu sequences, can hinder precise chromosomal pairing during mitosis and meiosis, resulting in unequal crossovers, one of the main reasons for chromosome duplication.

TEs use a number of different mechanisms to cause genetic instability and disease in their host genomes.

  • Expression of disease-causing, damaging proteins that inhibit normal cellular function.

Diseases

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Diseases often caused by TEs include

  • Hemophilia A and B
    • LINE1 (L1) TEs that land on the human Factor VIII have been shown to cause haemophilia[43]
  • Severe combined immunodeficiency
    • Insertion of L1 into the APC gene causes colon cancer, confirming that TEs play an important role in disease development.[44]
  • Porphyria
    • Insertion of Alu element into the PBGD gene leads to interference with the coding region and leads to acute intermittent porphyria[45] (AIP).
  • Predisposition to cancer
    • LINE1(L1) TE's and other retrotransposons have been linked to cancer because they cause genomic instability.[43]
  • Duchenne muscular dystrophy.[46][47]
    • Caused by SVA transposable element insertion in the fukutin (FKTN) gene which renders the gene inactive.[43]
  • Alzheimer's Disease and other Tauopathies
    • Transposable element dysregulation can cause neuronal death, leading to neurodegenerative disorders[48]

Rate of transposition, induction and defense

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One study estimated the rate of transposition of a particular retrotransposon, the Ty1 element in Saccharomyces cerevisiae. Using several assumptions, the rate of successful transposition event per single Ty1 element came out to be about once every few months to once every few years.[49] Some TEs contain heat-shock like promoters and their rate of transposition increases if the cell is subjected to stress,[50] thus increasing the mutation rate under these conditions, which might be beneficial to the cell.

Cells defend against the proliferation of TEs in a number of ways. These include piRNAs and siRNAs,[51] which silence TEs after they have been transcribed.

If organisms are mostly composed of TEs, one might assume that disease caused by misplaced TEs is very common, but in most cases TEs are silenced through epigenetic mechanisms like DNA methylation, chromatin remodeling and piRNA, such that little to no phenotypic effects nor movements of TEs occur as in some wild-type plant TEs. Certain mutated plants have been found to have defects in methylation-related enzymes (methyl transferase) which cause the transcription of TEs, thus affecting the phenotype.[6][52]

One hypothesis suggests that only approximately 100 LINE1 related sequences are active, despite their sequences making up 17% of the human genome. In human cells, silencing of LINE1 sequences is triggered by an RNA interference (RNAi) mechanism. Surprisingly, the RNAi sequences are derived from the 5′ untranslated region (UTR) of the LINE1, a long terminal which repeats itself. Supposedly, the 5′ LINE1 UTR that codes for the sense promoter for LINE1 transcription also encodes the antisense promoter for the miRNA that becomes the substrate for siRNA production. Inhibition of the RNAi silencing mechanism in this region showed an increase in LINE1 transcription.[6][53]

Evolution

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TEs are found in almost all life forms, and the scientific community is still exploring their evolution and their effect on genome evolution. It is unclear whether TEs originated in the last universal common ancestor, arose independently multiple times, or arose once and then spread to other kingdoms by horizontal gene transfer.[54] While some TEs confer benefits on their hosts, most are regarded as selfish DNA parasites. In this way, they are similar to viruses. Various viruses and TEs also share features in their genome structures and biochemical abilities, leading to speculation that they share a common ancestor.[55]

Because excessive TE activity can damage exons, many organisms have acquired mechanisms to inhibit their activity. Bacteria may undergo high rates of gene deletion as part of a mechanism to remove TEs and viruses from their genomes, while eukaryotic organisms typically use RNA interference to inhibit TE activity. Nevertheless, some TEs generate large families often associated with speciation events.[56] Evolution often deactivates DNA transposons, leaving them as introns (inactive gene sequences). In vertebrate animal cells, nearly all 100,000+ DNA transposons per genome have genes that encode inactive transposase polypeptides.[57] The first synthetic transposon designed for use in vertebrate (including human) cells, the Sleeping Beauty transposon system, is a Tc1/mariner-like transposon. Its dead ("fossil") versions are spread widely in the salmonid genome and a functional version was engineered by comparing those versions.[58] Human Tc1-like transposons are divided into Hsmar1 and Hsmar2 subfamilies. Although both types are inactive, one copy of Hsmar1 found in the SETMAR gene is under selection as it provides DNA-binding for the histone-modifying protein.[59] Many other human genes are similarly derived from transposons.[60] Hsmar2 has been reconstructed multiple times from the fossil sequences.[61]

The frequency and location of TE integrations influence genomic structure and evolution and affect gene and protein regulatory networks during development and in differentiated cell types.[62] Large quantities of TEs within genomes may still present evolutionary advantages, however. Interspersed repeats within genomes are created by transposition events accumulating over evolutionary time. Because interspersed repeats block gene conversion, they protect novel gene sequences from being overwritten by similar gene sequences and thereby facilitate the development of new genes. TEs may also have been co-opted by the vertebrate immune system as a means of producing antibody diversity. The V(D)J recombination system operates by a mechanism similar to that of some TEs. TEs also serve to generate repeating sequences that can form dsRNA to act as a substrate for the action of ADAR in RNA editing.[63]

TEs can contain many types of genes, including those conferring antibiotic resistance and the ability to transpose to conjugative plasmids. Some TEs also contain integrons, genetic elements that can capture and express genes from other sources. These contain integrase, which can integrate gene cassettes. There are over 40 antibiotic resistance genes identified on cassettes, as well as virulence genes.

Transposons do not always excise their elements precisely, sometimes removing the adjacent base pairs; this phenomenon is called exon shuffling. Shuffling two unrelated exons can create a novel gene product or, more likely, an intron.[64]

Some non-autonomous DNA TEs found in plants can capture coding DNA from genes and shuffle them across the genome.[65] This process can duplicate genes in the genome (a phenomenon called transduplication), and can contribute to generate novel genes by exon shuffling.[66]

Evolutionary drive for TEs on the genomic context

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There is a hypothesis that states that TEs might provide a ready source of DNA that could be co-opted by the cell to help regulate gene expression. Research showed that many diverse modes of TEs co-evolution along with some transcription factors targeting TE-associated genomic elements and chromatin are evolving from TE sequences. Most of the time, these particular modes do not follow the simple model of TEs and regulating host gene expression.[27]

Applications

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Main article: Transposons as a genetic tool

Transposable elements can be harnessed in laboratory and research settings to study genomes of organisms and even engineer genetic sequences. The use of transposable elements can be split into two categories: for genetic engineering and as a genetic tool.

Genetic engineering

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  • Insertional mutagenesis uses the features of a TE to insert a sequence. In most cases, this is used to remove a DNA sequence or cause a frameshift mutation.
    • In some cases the insertion of a TE into a gene can disrupt that gene's function in a reversible manner where transposase-mediated excision of the DNA transposon restores gene function.
    • This produces plants in which neighboring cells have different genotypes.
    • This feature allows researchers to distinguish between genes that must be present inside of a cell in order to function (cell-autonomous) and genes that produce observable effects in cells other than those where the gene is expressed.

Genetic tool

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In addition to the qualities mentioned for Genetic engineering, a Genetic tool also:-

  • Used for analysis of gene expression and protein functioning in signature-tagging mutagenesis.
    • This analytical tool allows researchers the ability to determine phenotypic expression of gene sequences. Also, this analytic technique mutates the desired locus of interest so that the phenotypes of the original and the mutated gene can be compared.

Specific applications

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  • TEs are also a widely used tool for mutagenesis of most experimentally tractable organisms. The Sleeping Beauty transposon system has been used extensively as an insertional tag for identifying cancer genes.[67]
  • The Tc1/mariner-class of TEs Sleeping Beauty transposon system, awarded Molecule of the Year in 2009,[68] is active in mammalian cells and is being investigated for use in human gene therapy.[69][70][71]
  • TEs are used for the reconstruction of phylogenies by the means of presence/absence analyses.[72] Transposons can act as biological mutagen in bacteria.
  • Common organisms which the use of Transposons has been well developed are:

De novo repeat identification

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De novo repeat identification is an initial scan of sequence data that seeks to find the repetitive regions of the genome, and to classify these repeats. Many computer programs exist to perform de novo repeat identification, all operating under the same general principles.[68] As short tandem repeats are generally 1–6 base pairs in length and are often consecutive, their identification is relatively simple.[67] Dispersed repetitive elements, on the other hand, are more challenging to identify, due to the fact that they are longer and have often acquired mutations. However, it is important to identify these repeats as they are often found to be transposable elements (TEs).[68]

De novo identification of transposons involves three steps: 1) find all repeats within the genome, 2) build a consensus of each family of sequences, and 3) classify these repeats. There are three groups of algorithms for the first step. One group is referred to as the k-mer approach, where a k-mer is a sequence of length k. In this approach, the genome is scanned for overrepresented k-mers; that is, k-mers that occur more often than is likely based on probability alone. The length k is determined by the type of transposon being searched for. The k-mer approach also allows mismatches, the number of which is determined by the analyst. Some k-mer approach programs use the k-mer as a base, and extend both ends of each repeated k-mer until there is no more similarity between them, indicating the ends of the repeats.[68] Another group of algorithms employs a method called sequence self-comparison. Sequence self-comparison programs use databases such as AB-BLAST to conduct an initial sequence alignment. As these programs find groups of elements that partially overlap, they are useful for finding highly diverged transposons, or transposons with only a small region copied into other parts of the genome.[69] Another group of algorithms follows the periodicity approach. These algorithms perform a Fourier transformation on the sequence data, identifying periodicities, regions that are repeated periodically, and are able to use peaks in the resultant spectrum to find candidate repetitive elements. This method works best for tandem repeats, but can be used for dispersed repeats as well. However, it is a slow process, making it an unlikely choice for genome-scale analysis.[68]

The second step of de novo repeat identification involves building a consensus of each family of sequences. A consensus sequence is a sequence that is created based on the repeats that comprise a TE family. A base pair in a consensus is the one that occurred most often in the sequences being compared to make the consensus. For example, in a family of 50 repeats where 42 have a T base pair in the same position, the consensus sequence would have a T at this position as well, as the base pair is representative of the family as a whole at that particular position, and is most likely the base pair found in the family's ancestor at that position.[68] Once a consensus sequence has been made for each family, it is then possible to move on to further analysis, such as TE classification and genome masking in order to quantify the overall TE content of the genome.

Adaptive TEs

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Transposable elements have been recognized as good candidates for stimulating gene adaptation, through their ability to regulate the expression levels of nearby genes.[70] Combined with their "mobility", transposable elements can be relocated adjacent to their targeted genes, and control the expression levels of the gene, dependent upon the circumstances.

The study conducted in 2008, "High Rate of Recent Transposable Element–Induced Adaptation in Drosophila melanogaster", used D. melanogaster that had recently migrated from Africa to other parts of the world, as a basis for studying adaptations caused by transposable elements. Although most of the TEs were located on introns, the experiment showed a significant difference in gene expressions between the population in Africa and other parts of the world. The four TEs that caused the selective sweep were more prevalent in D. melanogaster from temperate climates, leading the researchers to conclude that the selective pressures of the climate prompted genetic adaptation.[71] From this experiment, it has been confirmed that adaptive TEs are prevalent in nature, by enabling organisms to adapt gene expression as a result of new selective pressures.

However, not all effects of adaptive TEs are beneficial to the population. In the research conducted in 2009, "A Recent Adaptive Transposable Element Insertion Near Highly Conserved Developmental Loci in Drosophila melanogaster", a TE, inserted between Jheh 2 and Jheh 3, revealed a downgrade in the expression level of both of the genes. Downregulation of such genes has caused Drosophila to exhibit extended developmental time and reduced egg to adult viability. Although this adaptation was observed in high frequency in all non-African populations, it was not fixed in any of them.[72] This is not hard to believe, since it is logical for a population to favor higher egg to adult viability, therefore trying to purge the trait caused by this specific TE adaptation.

At the same time, there have been several reports showing the advantageous adaptation caused by TEs. In the research done with silkworms, "An Adaptive Transposable Element insertion in the Regulatory Region of the EO Gene in the Domesticated Silkworm", a TE insertion was observed in the cis-regulatory region of the EO gene, which regulates molting hormone 20E, and enhanced expression was recorded. While populations without the TE insert are often unable to effectively regulate hormone 20E under starvation conditions, those with the insert had a more stable development, which resulted in higher developmental uniformity.[74]

These three experiments all demonstrated different ways in which TE insertions can be advantageous or disadvantageous, through means of regulating the expression level of adjacent genes. The field of adaptive TE research is still under development and more findings can be expected in the future.

TEs participates in gene control networks

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Recent studies have confirmed that TEs can contribute to the generation of transcription factors. However, how this process of contribution can have an impact on the participation of genome control networks. TEs are more common in many regions of the DNA and it makes up 45% of total human DNA. Also, TEs contributed to 16% of transcription factor binding sites. A larger number of motifs are also found in non-TE-derived DNA, and the number is larger than TE-derived DNA. All these factors correlate to the direct participation of TEs in many ways of gene control networks.[27]

See also

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Notes

[edit]
  • Kidwell MG (2005). "Transposable elements". In T.R. Gregory (ed.). The Evolution of the Genome. San Diego: Elsevier. pp. 165–221. ISBN 978-0-123-01463-4.
  • Craig NL, Craigie R, Gellert M, and Lambowitz AM, eds. (2002). Mobile DNA II. Washington, DC: ASM Press. ISBN 978-1-555-81209-6.
  • Lewin B (2000). Genes VII. Oxford University Press. ISBN 978-0-198-79276-5.

References

[edit]
  1. ^ Bourque G, Burns KH, Gehring M, Gorbunova V, Seluanov A, Hammell M, et al. (November 2018). "Ten things you should know about transposable elements". Genome Biology. 19 (1): 199. doi:10.1186/s13059-018-1577-z. PMC 6240941. PMID 30454069.
  2. ^ Makałowski W, Gotea V, Pande A, Makałowska I (2019). "Transposable Elements: Classification, Identification, and Their Use as a Tool for Comparative Genomics". In Anisimova M (ed.). Evolutionary Genomics. Methods in Molecular Biology. Vol. 1910. New York, NY: Humana. pp. 185–186. doi:10.1007/978-1-4939-9074-0_6. ISBN 978-1-4939-9074-0. PMID 31278665. S2CID 195814061.
  3. ^ McClintock B (June 1950). "The origin and behavior of mutable loci in maize". Proceedings of the National Academy of Sciences of the United States of America. 36 (6): 344–55. Bibcode:1950PNAS...36..344M. doi:10.1073/pnas.36.6.344. PMC 1063197. PMID 15430309.
  4. ^ a b Wellinger, RE, et al. (2022). "A new challenge for data analytics: transposons". BioData Mining. 15 (9): 9. doi:10.1186/s13040-022-00294-x. PMC 8957154. PMID 35337342.
  5. ^ Bucher E, Reinders J, Mirouze M (November 2012). "Epigenetic control of transposon transcription and mobility in Arabidopsis". Current Opinion in Plant Biology. 15 (5): 503–10. Bibcode:2012COPB...15..503B. doi:10.1016/j.pbi.2012.08.006. PMID 22940592.
  6. ^ a b c Pray LA (2008). "Transposons: The jumping genes". Nature Education. 1 (1): 204.
  7. ^ a b c d e McGrayne SB (1998). Nobel Prize Women in Science: Their Lives, Struggles, and Momentous Discoveries (2nd ed.). Carol Publishing. p. 165. ISBN 978-0-9702256-0-3.
  8. ^ a b c McGrayne 1998, p. 166
  9. ^ McGrayne 1998, p. 167
  10. ^ McClintock B (November 1953). "Induction of Instability at Selected Loci in Maize". Genetics. 38 (6): 579–99. doi:10.1093/genetics/38.6.579. PMC 1209627. PMID 17247459.
  11. ^ Ravindran, S. (2012). "Proceedings of the National Academy of Sciences Dec 2012, 109 (50) 20198-20199; DOI: 10.1073/pnas.1219372109". Proceedings of the National Academy of Sciences of the United States of America. 109 (50): 20198–20199. doi:10.1073/pnas.1219372109. PMC 3528533. PMID 23236127.
  12. ^ Des Jardins J (2010). The Madame Curie Complex: The Hidden History of Women in Science. Feminist Press at CUNY. p. 246. ISBN 978-1-55861-655-4.
  13. ^ Fedoroff N, Botstein D, eds. (1 January 1992). The Dynamic Genome: Barbara McClintock's Ideas in the Century of Genetics. Cold Spring Harbor Laboratory Press. p. 2. ISBN 978-0-87969-422-7.
  14. ^ Kapitonov VV, Jurka J (May 2008). "A universal classification of eukaryotic transposable elements implemented in Repbase". Nature Reviews. Genetics. 9 (5): 411–2, author reply 414. doi:10.1038/nrg2165-c1. PMID 18421312. S2CID 1275744.
  15. ^ A not-so-selfish “genetic parasite” helps to preserve fertility
  16. ^ Walter M (2016). Transposon regulation upon dynamic loss of DNA methylation (Thesis). Université Pierre et Marie Curie. doi:10.13140/rg.2.2.18747.21286.
  17. ^ Madigan M, Martinko J, eds. (2006). Brock Biolog of Microorganisms (11th ed.). Prentice Hall. ISBN 978-0-13-144329-7.
  18. ^ a b Kazazian HH, Moran JV (May 1998). "The impact of L1 retrotransposons on the human genome". Nature Genetics. 19 (1): 19–24. doi:10.1038/ng0598-19. PMID 9590283. S2CID 33460203.
  19. ^ Capy P (1998). Dynamics and evolution of transposable elements. New York: Chapman & Hall. ISBN 978-3-540-61190-5.
  20. ^ Baez J (2005). "Subcellular Life Forms" (PDF).
  21. ^ Boutanaev AM, Osbourn AE (July 2018). "Multigenome analysis implicates miniature inverted-repeat transposable elements (MITEs) in metabolic diversification in eudicots". Proceedings of the National Academy of Sciences of the United States of America. 115 (28): E6650–E6658. Bibcode:2018PNAS..115E6650B. doi:10.1073/pnas.1721318115. PMC 6048515. PMID 29941591.
  22. ^ Kaminker JS, Bergman CM, Kronmiller B, Carlson J, Svirskas R, Patel S, Frise E, Wheeler DA, Lewis SE, Rubin GM, Ashburner M, Celniker SE (2002). "The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective". Genome Biology. 3 (12): RESEARCH0084. doi:10.1186/gb-2002-3-12-research0084. PMC 151186. PMID 12537573.
  23. ^ SanMiguel P, Tikhonov A, Jin YK, Motchoulskaia N, Zakharov D, Melake-Berhan A, et al. (November 1996). "Nested retrotransposons in the intergenic regions of the maize genome". Science. 274 (5288): 765–8. Bibcode:1996Sci...274..765S. doi:10.1126/science.274.5288.765. PMID 8864112. S2CID 33433647.
  24. ^ a b Jiao Y, Peluso P, Shi J, Liang T, Stitzer MC, Wang B, et al. (June 2017). "Improved maize reference genome with single-molecule technologies". Nature. 546 (7659): 524–527. Bibcode:2017Natur.546..524J. doi:10.1038/nature22971. PMC 7052699. PMID 28605751.
  25. ^ Mills RE, Bennett EA, Iskow RC, Devine SE (April 2007). "Which transposable elements are active in the human genome?". Trends in Genetics. 23 (4): 183–91. doi:10.1016/j.tig.2007.02.006. PMID 17331616.
  26. ^ Bruno M, Mahgoub M, Macfarlan TS (December 2019). "The Arms Race Between KRAB-Zinc Finger Proteins and Endogenous Retroelements and Its Impact on Mammals". Annual Review of Genetics. 53 (1). Annual Reviews: 393–416. doi:10.1146/annurev-genet-112618-043717. PMID 31518518. S2CID 202572327.
  27. ^ a b c d Zhou W, Liang G, Molloy PL, Jones PA (August 2020). "DNA methylation enables transposable element-driven genome expansion". Proceedings of the National Academy of Sciences of the United States of America. 117 (32): 19359–19366. Bibcode:2020PNAS..11719359Z. doi:10.1073/pnas.1921719117. PMC 7431005. PMID 32719115.
  28. ^ Plant Transposable Elements, ed. Nelson (Plenum Publishing, 1988), pp. 161–174.
  29. ^ Nowacki M, Higgins BP, Maquilan GM, Swart EC, Doak TG, Landweber LF (May 2009). "A functional role for transposases in a large eukaryotic genome". Science. 324 (5929): 935–8. Bibcode:2009Sci...324..935N. doi:10.1126/science.1170023. PMC 3491810. PMID 19372392.
  30. ^ Spradling AC, Rubin GM (October 1982). "Transposition of cloned P elements into Drosophila germ line chromosomes". Science. 218 (4570): 341–7. Bibcode:1982Sci...218..341S. doi:10.1126/science.6289435. PMID 6289435.
  31. ^ Rubin GM, Spradling AC (October 1982). "Genetic transformation of Drosophila with transposable element vectors". Science. 218 (4570): 348–53. Bibcode:1982Sci...218..348R. doi:10.1126/science.6289436. PMID 6289436.
  32. ^ Cesari F (15 October 2007). "Milestones in Nature: Milestone 9: Transformers, Elements in Disguise". Nature. 8: S10. doi:10.1038/nrg2254.
  33. ^ Jacobson JW, Medhora MM, Hartl DL (November 1986). "Molecular structure of a somatically unstable transposable element in Drosophila". Proceedings of the National Academy of Sciences of the United States of America. 83 (22): 8684–8. Bibcode:1986PNAS...83.8684J. doi:10.1073/pnas.83.22.8684. PMC 386995. PMID 3022302.
  34. ^ Lohe AR, Moriyama EN, Lidholm DA, Hartl DL (January 1995). "Horizontal transmission, vertical inactivation, and stochastic loss of mariner-like transposable elements". Molecular Biology and Evolution. 12 (1): 62–72. doi:10.1093/oxfordjournals.molbev.a040191. PMID 7877497.
  35. ^ Lampe DJ, Witherspoon DJ, Soto-Adames FN, Robertson HM (April 2003). "Recent horizontal transfer of mellifera subfamily mariner transposons into insect lineages representing four different orders shows that selection acts only during horizontal transfer". Molecular Biology and Evolution. 20 (4): 554–62. doi:10.1093/molbev/msg069. PMID 12654937.
  36. ^ Mandal PK, Kazazian HH (October 2008). "SnapShot: Vertebrate transposons". Cell. 135 (1): 192–192.e1. doi:10.1016/j.cell.2008.09.028. PMID 18854165. S2CID 82147.
  37. ^ Carlton JM, Hirt RP, Silva JC, Delcher AL, Schatz M, Zhao Q, et al. (January 2007). "Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalis". Science. 315 (5809): 207–12. Bibcode:2007Sci...315..207C. doi:10.1126/science.1132894. PMC 2080659. PMID 17218520.
  38. ^ Kim JM, Vanguri S, Boeke JD, Gabriel A, Voytas DF (May 1998). "Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence". Genome Research. 8 (5): 464–78. doi:10.1101/gr.8.5.464. PMID 9582191.
  39. ^ Van't Hof, Arjen E.; Campagne, Pascal; Rigden, Daniel J. (1 June 2016). "The industrial melanism mutation in British peppered moths is a transposable element". Nature. 534 (7605). Springer: 102–105. Bibcode:2016Natur.534..102H. doi:10.1038/nature17951. ISSN 1476-4687. PMID 27251284. S2CID 3989607.
  40. ^ Cosby, Rachel L.; Chang, Ni-Chen; Feschotte, Cédric (1 September 2019). "Host–transposon interactions: conflict, cooperation, and cooption". Genes & Development. 33 (17–18). Cold Spring Harbor Laboratory Press & The Genetics Society: 1098–1116. doi:10.1101/gad.327312.119. ISSN 0890-9369. PMC 6719617. PMID 31481535.
  41. ^ a b Belancio VP, Hedges DJ, Deininger P (March 2008). "Mammalian non-LTR retrotransposons: for better or worse, in sickness and in health". Genome Research. 18 (3): 343–58. doi:10.1101/gr.5558208. PMID 18256243.
  42. ^ Dahlet T, Argüeso Lleida A, Al Adhami H, Dumas M, Bender A, Ngondo RP, et al. (June 2020). "Genome-wide analysis in the mouse embryo reveals the importance of DNA methylation for transcription integrity". Nature Communications. 11 (1): 3153. Bibcode:2020NatCo..11.3153D. doi:10.1038/s41467-020-16919-w. PMC 7305168. PMID 32561758.
  43. ^ a b c Kazazian HH, Wong C, Youssoufian H, Scott AF, Phillips DG, Antonarakis SE (March 1988). "Haemophilia A resulting from de novo insertion of L1 sequences represents a novel mechanism for mutation in man". Nature. 332 (6160): 164–6. Bibcode:1988Natur.332..164K. doi:10.1038/332164a0. PMID 2831458.
  44. ^ Miki Y, Nishisho I, Horii A, Miyoshi Y, Utsunomiya J, Kinzler KW, Vogelstein B, Nakamura Y (February 1992). "Disruption of the APC gene by a retrotransposal insertion of L1 sequence in a colon cancer". Cancer Research. 52 (3): 643–5. PMID 1310068.
  45. ^ Mustajoki S, Ahola H, Mustajoki P, Kauppinen R (June 1999). "Insertion of Alu element responsible for acute intermittent porphyria". Human Mutation. 13 (6): 431–8. doi:10.1002/(sici)1098-1004(1999)13:6<431::aid-humu2>3.0.co;2-y. PMID 10408772. S2CID 6218429.
  46. ^ Kazazian HH, Goodier JL (August 2002). "LINE drive. retrotransposition and genome instability". Cell. 110 (3): 277–80. doi:10.1016/S0092-8674(02)00868-1. PMID 12176313.
  47. ^ Kapitonov VV, Pavlicek A, Jurka J (2006). Anthology of Human Repetitive DNA. Encyclopedia of Molecular Cell Biology and Molecular Medicine. doi:10.1002/3527600906.mcb.200300166. ISBN 978-3527600908.
  48. ^ Sun W, Samimi H, Gamez M, Zare H, Frost B (August 2018). "Pathogenic tau-induced piRNA depletion promotes neuronal death through transposable element dysregulation in neurodegenerative tauopathies". Nature Neuroscience. 21 (8): 1038–1048. doi:10.1038/s41593-018-0194-1. PMC 6095477. PMID 30038280.
  49. ^ Paquin CE, Williamson VM (October 1984). "Temperature effects on the rate of ty transposition". Science. 226 (4670): 53–5. Bibcode:1984Sci...226...53P. doi:10.1126/science.226.4670.53. PMID 17815421. S2CID 39145808.
  50. ^ Strand DJ, McDonald JF (June 1985). "Copia is transcriptionally responsive to environmental stress". Nucleic Acids Research. 13 (12): 4401–10. doi:10.1093/nar/13.12.4401. PMC 321795. PMID 2409535.
  51. ^ Chung WJ, Okamura K, Martin R, Lai EC (June 2008). "Endogenous RNA interference provides a somatic defense against Drosophila transposons". Current Biology. 18 (11): 795–802. Bibcode:2008CBio...18..795C. doi:10.1016/j.cub.2008.05.006. PMC 2812477. PMID 18501606.
  52. ^ a b Miura A, Yonebayashi S, Watanabe K, Toyama T, Shimada H, Kakutani T (May 2001). "Mobilization of transposons by a mutation abolishing full DNA methylation in Arabidopsis". Nature. 411 (6834): 212–4. Bibcode:2001Natur.411..212M. doi:10.1038/35075612. PMID 11346800. S2CID 4429219.
  53. ^ Yang N, Kazazian HH (September 2006). "L1 retrotransposition is suppressed by endogenously encoded small interfering RNAs in human cultured cells". Nature Structural & Molecular Biology. 13 (9): 763–71. doi:10.1038/nsmb1141. PMID 16936727. S2CID 32601334.
  54. ^ Kidwell MG (1992). "Horizontal transfer of P elements and other short inverted repeat transposons". Genetica. 86 (1–3): 275–86. doi:10.1007/BF00133726. PMID 1334912. S2CID 33227644.
  55. ^ Villarreal L (2005). Viruses and the Evolution of Life. Washington: ASM Press.
  56. ^ Ricci, Marco; Peona, Valentina; Guichard, Etienne; Taccioli, Cristian; Boattini, Alessio (31 May 2018). "Transposable Elements Activity is Positively Related to Rate of Speciation in Mammals". Journal of Molecular Evolution. 86 (5): 303–310. Bibcode:2018JMolE..86..303R. doi:10.1007/s00239-018-9847-7. PMC 6028844. PMID 29855654.
  57. ^ Plasterk RH, Izsvák Z, Ivics Z (August 1999). "Resident aliens: the Tc1/mariner superfamily of transposable elements". Trends in Genetics. 15 (8): 326–32. doi:10.1016/S0168-9525(99)01777-1. PMID 10431195.
  58. ^ Ivics Z, Hackett PB, Plasterk RH, Izsvák Z (November 1997). "Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells". Cell. 91 (4): 501–10. doi:10.1016/S0092-8674(00)80436-5. PMID 9390559. S2CID 17908472.
  59. ^ Miskey C, Papp B, Mátés L, Sinzelle L, Keller H, Izsvák Z, Ivics Z (June 2007). "The ancient mariner sails again: transposition of the human Hsmar1 element by a reconstructed transposase and activities of the SETMAR protein on transposon ends". Molecular and Cellular Biology. 27 (12): 4589–600. doi:10.1128/MCB.02027-06. PMC 1900042. PMID 17403897.
  60. ^ "Gene group: Transposable element derived genes". HUGO Gene Nomenclature Committee. Retrieved 4 March 2019.
  61. ^ Gil E, Bosch A, Lampe D, Lizcano JM, Perales JC, Danos O, Chillon M (11 September 2013). "Functional characterization of the human mariner transposon Hsmar2". PLOS ONE. 8 (9): e73227. Bibcode:2013PLoSO...873227G. doi:10.1371/journal.pone.0073227. PMC 3770610. PMID 24039890.
  62. ^ Ball, Hope C.; Ansari, Mohammad Y.; Ahmad, Nashrah; Novak, Kimberly; Haqqi, Tariq M. (November 2021). "A retrotransposon gag-like-3 gene RTL3 and SOX-9 co-regulate the expression of COL2A1 in chondrocytes". Connective Tissue Research. 62 (6): 615–628. doi:10.1080/03008207.2020.1828380. ISSN 1607-8438. PMC 8404968. PMID 33043724.
  63. ^ Jin Y, Zhang W, Li Q (June 2009). "Origins and evolution of ADAR-mediated RNA editing". IUBMB Life. 61 (6): 572–578. doi:10.1002/iub.207. PMID 19472181.
  64. ^ Moran JV, DeBerardinis RJ, Kazazian HH (March 1999). "Exon shuffling by L1 retrotransposition". Science. 283 (5407): 1530–4. Bibcode:1999Sci...283.1530M. doi:10.1126/science.283.5407.1530. PMID 10066175.
  65. ^ Jiang N, Bao Z, Zhang X, Eddy SR, Wessler SR (September 2004). "Pack-MULE transposable elements mediate gene evolution in plants". Nature. 431 (7008): 569–573. Bibcode:2004Natur.431..569J. doi:10.1038/nature02953. PMID 15457261. S2CID 4363679.
  66. ^ Catoni M, Jonesman T, Cerruti E, Paszkowski J (February 2019). "Mobilization of Pack-CACTA transposons in Arabidopsis suggests the mechanism of gene shuffling". Nucleic Acids Research. 47 (3): 1311–1320. doi:10.1093/nar/gky1196. PMC 6379663. PMID 30476196.
  67. ^ a b Saha S, Bridges S, Magbanua ZV, Peterson DG (2008). "Computational Approaches and Tools Used in Identification of Dispersed Repetitive DNA Sequences". Tropical Plant Biol. 1: 85–96. doi:10.1007/s12042-007-9007-5. S2CID 26272439.
  68. ^ a b c d e f Makałowski W, Pande A, Gotea V, Makałowska I (2012). "Transposable elements and their identification". Evolutionary Genomics. Methods in Molecular Biology. Vol. 855. pp. 337–59. doi:10.1007/978-1-61779-582-4_12. ISBN 978-1-61779-581-7. PMID 22407715.
  69. ^ a b Saha S, Bridges S, Magbanua ZV, Peterson DG (April 2008). "Empirical comparison of ab initio repeat finding programs". Nucleic Acids Research. 36 (7): 2284–94. doi:10.1093/nar/gkn064. PMC 2367713. PMID 18287116.
  70. ^ a b Mariño-Ramírez L, Lewis KC, Landsman D, Jordan IK (2005). "Transposable elements donate lineage-specific regulatory sequences to host genomes". Cytogenetic and Genome Research. 110 (1–4): 333–41. doi:10.1159/000084965. PMC 1803082. PMID 16093685.
  71. ^ a b González J, Lenkov K, Lipatov M, Macpherson JM, Petrov DA (October 2008). "High rate of recent transposable element-induced adaptation in Drosophila melanogaster". PLOS Biology. 6 (10): e251. doi:10.1371/journal.pbio.0060251. PMC 2570423. PMID 18942889.
  72. ^ a b González J, Macpherson JM, Petrov DA (September 2009). "A recent adaptive transposable element insertion near highly conserved developmental loci in Drosophila melanogaster". Molecular Biology and Evolution. 26 (9): 1949–61. doi:10.1093/molbev/msp107. PMC 2734154. PMID 19458110.
  73. ^ Tempel S, Rousseau C, Tahi F, Nicolas J (September 2010). "ModuleOrganizer: detecting modules in families of transposable elements". BMC Bioinformatics. 11: 474. doi:10.1186/1471-2105-11-474. PMC 2955051. PMID 20860790.
  74. ^ Sun W, Shen YH, Han MJ, Cao YF, Zhang Z (December 2014). "An adaptive transposable element insertion in the regulatory region of the EO gene in the domesticated silkworm, Bombyx mori". Molecular Biology and Evolution. 31 (12): 3302–13. doi:10.1093/molbev/msu261. PMID 25213334.
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